KMID : 0380020130280020086
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Korean Journal of Biotechnology and Bioengineering 2013 Volume.28 No. 2 p.86 ~ p.91
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Effects of Silkworm Gland Hydrolysate on Albumin-erythropoietin Production in Transgenic Chinese Hamster Ovary Cells
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Choi Min-Ho
Cha Hyun-Myoung Kim Sun-Mi Choi Yong-Soo Kim Dong-Il
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Abstract
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To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb-EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26- fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.
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KEYWORD
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Albumin-EPO, Apoptosis, CHO cell, Silkworm Gland Hydrolysate
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